p40px egfp sequence (Addgene inc)
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p40px egfp sequence
P40px Egfp Sequence, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/p40px+egfp+sequence/p40PX-EGFP+(Plasmid+%2319010)/bio_rxiv__64898__2025__12__16__694573-154-13-15
Average 93 stars, based on 23 article reviews
P40px Egfp Sequence, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/p40px+egfp+sequence/p40PX-EGFP+(Plasmid+%2319010)/bio_rxiv__64898__2025__12__16__694573-154-13-15
Average 93 stars, based on 23 article reviews
p40px egfp sequence - by Bioz Stars,
2026-09
93/100 stars
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Construct:Article Title: LRRK2 is activated by phosphatidylinositol 3-phosphate in conjunction with CASM Article Snippet: To construct plasmids encoding 3×FLAG-human LRRK2 with an alanine substitution mutation at the N-terminal region, p3×FLAG-human LRRK2 WT were first cut by Bsp1407 I (Takara Bio) to remove LRRK2 N-terminal region, and then the two PCR amplicons overlapping the mutation site as well as the cleaved plasmid were assembled using NEBuilder (New England Biolabs). .. To construct a lentiviral plasmid for the expression of p40PX-EGFP in RAW264.7 cells, Plasmid Preparation:Article Title: LRRK2 is activated by phosphatidylinositol 3-phosphate in conjunction with CASM Article Snippet: To construct plasmids encoding 3×FLAG-human LRRK2 with an alanine substitution mutation at the N-terminal region, p3×FLAG-human LRRK2 WT were first cut by Bsp1407 I (Takara Bio) to remove LRRK2 N-terminal region, and then the two PCR amplicons overlapping the mutation site as well as the cleaved plasmid were assembled using NEBuilder (New England Biolabs). .. To construct a lentiviral plasmid for the expression of p40PX-EGFP in RAW264.7 cells, Expressing:Article Title: LRRK2 is activated by phosphatidylinositol 3-phosphate in conjunction with CASM Article Snippet: To construct plasmids encoding 3×FLAG-human LRRK2 with an alanine substitution mutation at the N-terminal region, p3×FLAG-human LRRK2 WT were first cut by Bsp1407 I (Takara Bio) to remove LRRK2 N-terminal region, and then the two PCR amplicons overlapping the mutation site as well as the cleaved plasmid were assembled using NEBuilder (New England Biolabs). .. To construct a lentiviral plasmid for the expression of p40PX-EGFP in RAW264.7 cells, Sequencing:Article Title: LRRK2 is activated by phosphatidylinositol 3-phosphate in conjunction with CASM Article Snippet: To construct plasmids encoding 3×FLAG-human LRRK2 with an alanine substitution mutation at the N-terminal region, p3×FLAG-human LRRK2 WT were first cut by Bsp1407 I (Takara Bio) to remove LRRK2 N-terminal region, and then the two PCR amplicons overlapping the mutation site as well as the cleaved plasmid were assembled using NEBuilder (New England Biolabs). .. To construct a lentiviral plasmid for the expression of p40PX-EGFP in RAW264.7 cells, Polymerase Chain Reaction:Article Title: LRRK2 is activated by phosphatidylinositol 3-phosphate in conjunction with CASM Article Snippet: To construct plasmids encoding 3×FLAG-human LRRK2 with an alanine substitution mutation at the N-terminal region, p3×FLAG-human LRRK2 WT were first cut by Bsp1407 I (Takara Bio) to remove LRRK2 N-terminal region, and then the two PCR amplicons overlapping the mutation site as well as the cleaved plasmid were assembled using NEBuilder (New England Biolabs). .. To construct a lentiviral plasmid for the expression of p40PX-EGFP in RAW264.7 cells, Ligation:Article Title: LRRK2 is activated by phosphatidylinositol 3-phosphate in conjunction with CASM Article Snippet: To construct plasmids encoding 3×FLAG-human LRRK2 with an alanine substitution mutation at the N-terminal region, p3×FLAG-human LRRK2 WT were first cut by Bsp1407 I (Takara Bio) to remove LRRK2 N-terminal region, and then the two PCR amplicons overlapping the mutation site as well as the cleaved plasmid were assembled using NEBuilder (New England Biolabs). .. To construct a lentiviral plasmid for the expression of p40PX-EGFP in RAW264.7 cells, |